Easy DIY Buttermilk Using Lemon Juice
Here is how to make buttermilk using lemon juice. Sometimes you want to make a recipe and realize you need buttermilk but don’t have any at home. Instead of running to the store, you can make your own buttermilk substitute in just a few minutes. Learning how to make buttermilk with lemon juice is easy and gives you the same tangy flavor that recipes like pancakes, muffins, biscuits, and cakes need.
This homemade buttermilk recipe only takes two ingredients. Milk and lemon juice. It works just as well as store bought buttermilk. Knowing how to make your own buttermilk is a great kitchen trick to keep on hand whenever you’re out.

This is the recipe I’ve been using for years when I don’t have buttermilk on hand. Just a little lemon juice and milk makes the perfect swap, and it’s so easy to mix up in a few minutes. I’ve used it in pancakes, muffins, and breads, and it always gives that same rich flavor that real buttermilk does.
What Is Buttermilk?
Buttermilk is a cultured dairy product made by adding live bacteria to milk, which creates a slightly thick texture and tangy flavor. It’s often used in baking because the acidity helps activate baking soda, giving baked goods a light and fluffy texture. Buttermilk also adds moisture and a rich taste to recipes like pancakes, biscuits, muffins, and breads. Today, most buttermilk you find in stores is cultured buttermilk, which is different from the traditional liquid left after churning butter.

How To Make Buttermilk With Lemon Juice
Ingredients For DIY Buttermilk
You can use any type of milk for this recipe, but fuller fat milks work best.
- 1 cup of milk
- 1 tbsp lemon juice

Instructions For DIY Buttermilk
Step 1
Measure the milk: Pour 1 cup of milk into a measuring cup.
Step 2
Add the lemon juice: Stir in 1 tablespoon of fresh lemon juice.
Step 3
Let it rest: Leave the mixture at room temperature for 20 minutes until it thickens and curdles slightly. Some recipes for DIY buttermilk says it’s ready after about 10 minutes, but I like to leave mine for a solid 20 minutes so it thickens up really well.
DIY Buttermilk FAQ
Can I use vinegar instead of lemon juice for buttermilk?
Yes, white vinegar works the same way as lemon juice to make buttermilk. Just use 1 tablespoon of vinegar for every 1 cup of milk.
What type of milk is best for homemade buttermilk?
Whole milk gives the richest flavor and texture, but you can use 2% or even skim if that’s what you have. The consistency may be a little thinner with low fat milk. I find that fuller fat milk works best for a thick buttermilk.
How long does homemade buttermilk last?
Homemade buttermilk is best used right away, but you can store it in the fridge for up to 2 days. Make sure to stir before using as it may separate.

Can I use plant-based milk to make buttermilk?
Yes, you can use milks like almond milk with lemon juice or vinegar to create a similar effect. It won’t be as creamy as dairy buttermilk, but it still works in recipes.
Why does buttermilk make baked goods fluffy?
The acid in buttermilk reacts with baking soda, creating bubbles that help batter rise. This gives cakes, pancakes, and muffins a soft and airy texture.
Tips For Making Buttermilk With Lemon Juice
Use fresh milk
Always start with fresh milk for the best flavor and consistency. Whole milk gives a richer texture, but 2% or skim milk works in a pinch.
Measure the acid carefully
Use 1 tablespoon of lemon juice or white vinegar per cup of milk. This give the right balance to create a tangy buttermilk without curdling too much.
Let it sit
After adding the acid, let the mixture rest for 20 minutes at room temperature. This allows the milk to thicken slightly and develop that characteristic tangy flavor.
Stir before using
Give the mixture a quick stir before adding it to your recipe. This helps recombine any separation and ensures the buttermilk is evenly mixed.
Make it fresh
Homemade buttermilk works best right after it’s made. Using it immediately guarantees the proper acidity and texture for baking, pancakes, muffins, and more.
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